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eif4a inhibitor cr 1 31 b  (MedChemExpress)


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    MedChemExpress eif4a inhibitor cr 1 31 b
    PDCD4 regulates the secretion of M2‐polarizing proteins in an <t>eIF4A‐dependent</t> manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Eif4a Inhibitor Cr 1 31 B, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eif4a inhibitor cr 1 31 b/product/MedChemExpress
    Average 93 stars, based on 12 article reviews
    eif4a inhibitor cr 1 31 b - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma Dedifferentiation"

    Article Title: Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma Dedifferentiation

    Journal: Advanced Science

    doi: 10.1002/advs.202506925

    PDCD4 regulates the secretion of M2‐polarizing proteins in an eIF4A‐dependent manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: PDCD4 regulates the secretion of M2‐polarizing proteins in an eIF4A‐dependent manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Expressing, Co-Immunoprecipitation Assay, Knock-Out, Over Expression, Knockdown, Inhibition, In Vivo, Immunoprecipitation, Flow Cytometry, Western Blot, Comparison



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    eif4a inhibitor cr 1 31 b  (MedChemExpress)
    93
    MedChemExpress eif4a inhibitor cr 1 31 b
    PDCD4 regulates the secretion of M2‐polarizing proteins in an <t>eIF4A‐dependent</t> manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Eif4a Inhibitor Cr 1 31 B, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eif4a inhibitor cr 1 31 b/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    eif4a inhibitor cr 1 31 b - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

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    PDCD4 regulates the secretion of M2‐polarizing proteins in an eIF4A‐dependent manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Advanced Science

    Article Title: Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma Dedifferentiation

    doi: 10.1002/advs.202506925

    Figure Lengend Snippet: PDCD4 regulates the secretion of M2‐polarizing proteins in an eIF4A‐dependent manner. A) Schematic diagram of PDCD4–eIF4A‐mediated regulation of protein expression patterns. B) Co‐IP analysis showing the changes in the eIF4F complex (eIF4A–eIF4E–eIF4G) upon PDCD4 knockout or overexpression in BHT101 cells. C) WB analysis confirmed the knockdown efficiency of eIF4A using siRNA in TPC‐sg‐PDCD4 and BHT101. D,E) FCM analysis showing that inhibition of eIF4A reduced M2 macrophage polarization induced by TPC‐sg‐PDCD4 (D) and BHT101 (E) cells in THP‐1 polarization assays. F) In vivo experiments showed that the eIF4A inhibitor suppressed subcutaneous tumor growth in mice. G,H) FCM revealed that eIF4A inhibition decreased M2 macrophage infiltration (G) and increased M1 macrophage infiltration (H) in tumors. I–L) Proteomic and pathway enrichment analyses of intracellular proteins (I, K) and secreted proteins in the supernatant (J, L) after PDCD4 knockout in TPC‐1 cells. M,N) Polysome profiling analysis demonstrated that PDCD4 knockout affected the translational efficiency of specific mRNAs in TPC‐1 cells. Co‐IP : Co‐immunoprecipitation ; eIF4A : eukaryotic Initiation Factor 4A ; eIF4E : eukaryotic Initiation Factor 4E ; eIF4G : eukaryotic Initiation Factor 4G ; FCM : Flow cytometry; PDCD4 : Programmed Cell Death 4; WB : Western Blot. Significance in D‐F was determined using one‐way ANOVA and the Bonferroni multiple comparison test. Significance in G, H was determined using Unpaired t‐test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: The eIF4A inhibitor CR‐1‐31‐B (MCE, Cat#HY‐136453) was administered to mice at a dose of 2 mg kg −1 via intraperitoneal injection every other day in mouse drug administration experiments.

    Techniques: Expressing, Co-Immunoprecipitation Assay, Knock-Out, Over Expression, Knockdown, Inhibition, In Vivo, Immunoprecipitation, Flow Cytometry, Western Blot, Comparison